sticky end(cohesive end)
7.The plant expression vector pBI121. 2 was digested with BamH I and Sma I , the bovine casein B gene cDNA code fragment was seperated from pBα slC184 plasmid with Bgl Ⅱ and EcoR I, the cloning process includes two steps: the first, the Bgl Ⅱ sticky end of casein B gene was ligased to BamH Ⅲ sticky end of pBI 121. 2;


