strain step
2.The analysis of the distributions of simulated strain and velocity shows that the fins are multi-step local deformed.
3.The advance of the metabolic engineering was briefly reviewed and the application of metabolic engineering methods for the construction of D-ribose-producing genetic engineering strain such as host selection, rate-limited step acceleration, metabolic flow alteration, fermentation process optimization and its application prospects of metabolic engineering was also described.
4.Aeromonas sobria YH311 was adopted as a starting strain, crude chondroitinase was initially isolated with ammonium sulfate precipitation from cultured liquid. Then the crude chondroitinase was dissolved and dialyzed, and again to purify step-by-step through CM-cellulose, QAE-Sephadex A50 an Sephadex G-150 chromatography, at the same time its enzyme activity was track tested. Finally, chondroitinase was obtained and exhibited single band on SDS-PAGE testing.
5.The manufacturing process for VDC/VC polyvinylidene chloride film was simulated step by step to analyze the effect of processing parameters, including temperature, strain and time, on the crystallization. Some analyzing techniques like X ray diffraction and DSC were applied to observing the crystal structure of samples.
6.Methods One-step knock-out of chromosomal method was used to disrupt chromosomal genes, in Salmonella typhimurium wild strain X2. The acrAB gene and tolC gene were knocked out and two mutants of the △acrABX2 and △tolCX2 were produced.
8.Methods Cultured dermal fibroblasts were treated by 8 methoxypsoralen /ultraviolet A (8 MOP/UVA). mtDNA was extracted by one step method and the PCR products of D loop and adjacent transcription promoter 6 (DLP 6) fragment of mtDNA control region for replication were detected by polymerase chain reaction single strain conformation polymorphism and direct sequencing.
9.The explicit finite element method(FEM) based on the varying time-step was selected to analyse the dynamic roll-pass process; the nodes on the leading face of the steady-state elements set was extracted and projected onto a plane to give the required mesh, also called the origin mesh; the implicit FEM was selected to analyses the inter-pass transient thermal process; a geometric part from the mesh was generated and meshed by using the quadrilateral elements, a heat transfer analysis was selected to transfer the node temperature data; a new model was created by using hexahedral elements, mapping the node temperature and the equivalent plastic strain on the integration points.
