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1.Methods Mice were immunized with bovine submaxillary mucin (BSM), the splenic lymphocytes from the immunized mouse were fused with the mouse myeloma Sp2/0 cells using PEG.
方法 以牛颌下腺粘蛋白(BSM)为免疫原免疫小鼠,以免疫脾细胞与Sp2/0细胞融合建立杂交瘤细胞株,用有限稀释法克隆化,间接ELISA及间接ELISA结合神经氨酸酶消化筛选克隆。收藏指正
2.Aim To determine the thermodynamics of binding reaction of arginine oligomer (R8) to bovine submaxillary mucin (BSM) in order to provide the foundation for understanding the influence of mucin on transport of macromolecules through mucosa mediated by arginine oligomer.
目的 通过测定精氨酸八聚体(R8)与牛颌下腺粘蛋白(BSM)结合反应的热力学参数,了解粘蛋白影响 寡聚精氨酸促进生物大分子跨黏膜转运的可能机制。收藏指正
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名词复数:submaxillaries
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