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1.In addition, two different bases in 0-A EIAV TAR element were mutated to the same asthose of DLA EIAV TAR by PCR-directed mutagenesis. Namely, two adenines (A) locatedwithin 0-A EIAV TAR element and its neighbor region, respectively, were mutated to guanine(G) and thymine (T).
此外,本研究还通过PCR定点突变技术将D-A EIAV TAR元件中的差异碱基按照强毒致弱过程中的改变进行了突变,即将D-A EIAV TAR中的碱基A突变为DLA EIAV相同的碱基G,同时将D-A EIAV TAR相邻区域+3位的碱基A定点突变为与DLA EIAV相同的T。收藏指正
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