transcription-polymerse
1.Methods Reverse transcription-polymerse chain reaction and ELSA were used to detect the gene phenotype and the expression of hyaluronidase.
2.Methods Reverse transcription and polymerse chain reaction (RT-PCR)was used to detect the expression of gax mRNA and immunohistochemistry straining was per- formed to examine the expression of PCNA protein in splenic vein of 28 patients with portal hypertension (experimental group)and 12 normal vasculatures(control group).
