translation initiation
1.Effect of La Protein and Its Mutants on the Transcription Translation Initiation and Replication of Hepatitis B Virus
2.Based on the position weight matrix(PWM) and length distribution of open reading frame(ORF),a simple method for predicting translation initiation sites is presented. It can identify TIS from upstream AUGs easily.
3.Methods The DNA fregment target to the translation initiation site of CCR5 mRNA obtained by RT - PCR from peripheral blood mononuclear cells(PBMCs)was cloned into retro-viral vector pLXSN.
4.ramosissima include calcium-dependent protein kinase 3, calmodulin, manganese superoxide dismutase, glutathione reductase, dehydration-responsive protein RD22, drought-induced proteinl, eukaryotic translation initiation factor 5A-2, ubiquitin-conjugating protein, malate dehydrogenase and S-adenosylmethionine synthetase, ect.
5.Methods: A 12 mer phosphorothioate TFO (TFO12) directed at the second transcriotion start site of N-ras gene and a 19 mer phosphorothioate ODNas (ODNas19) complementary to translation initiation codon and downstream 5 codons of N-ras mRNA were synthesized.
6.Results:Treatment with two oligonucleotides directed against the coding region and the translation initiation region of bcl 2 messenger RNA/VP 16 combination respectively for 48 h had significantly reduced the number of viable AL cells.
7.MALDI-TOF-MS showed that the highly expressed proteins were: an unknown protein,proliferation-associated gene A,Up1,alternative splicing factor ASF-3,cofilin1(non-muscle),eukaryotic translation initiation factor 5A,beta galactoside binding lectin,and glutathione-S-transferase Pi.
8.To examine of the effects of mRNA secondary structures in the TIR(translation initiation region) on the translation efficiency of foreign genes in COS-7 cells,four consecutive identical codons(6×ATT,3×ATT,6×GCC and 3×GCC) were introduced into luciferase gene downstream AUG to produce four luciferase gene variants with different TIR secondary structures. Then they were transfected into COS-7 cells.
9.Background & Objective: The previous study has identified two novel antisense oligonucleotides(AS ODN) of new target point in the translation initiation and the coding region of bcl 2 mRNA that could increase the sensitivity of HL 60 and K562 cell lines to etoposide, daunorubicin,and araninosyl cytosine (Ara C).
