1.Leukemic colony formation unit (L-CFU) was cultured in soft agarmedium in 10 cases with acute myeloblastic leukemia (AML) with PHA-LCM condition medium as a stimulating factor.
2.Results:1.Solid cultivation medium for Hp: Colombian agarmedium with mixed antibiotic added with 6% sheep blood and 6% horse serum,respectively if under 37℃ microaeropgilic condition,after 3 days,could cultivate Hp;
6.The best root differentiation medium is WPM supplemented with 0.4mg/L IBA, 5.6g/L agar; the best leaf regeneration medium is MS supplemented with 0.5mg/L 6-BA, 0.1mg/L NAA;