tumour explant culture
2.Result shew: the number of chondrocyte of enzyme digestion was more than that of explant culture,The viability of chondrocytes isolated with collagenase typeⅡconfected by serumfree medium were markedly higher than that of PBS (free of Ca~ 2+ ,Mg~ 2+ ). There was statistical discrepancy between the two enzyme digestion groups(P<0.05).


