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1.Epidermal explant culture was applied, and effects of AK auto Ab with variable dose on rate of epidermaration were observed. Brd U incorporation assay was used to investigate the extent of mitosis.
方法 采用贴壁法体外培养皮肤 ,比较观察不同浓度 AK auto Ab对表皮形成速率的影响 ,并采用 Brd- U掺入法测定各组细胞增殖率 .收藏指正
2.Result shew: the number of chondrocyte of enzyme digestion was more than that of explant culture,The viability of chondrocytes isolated with collagenase typeⅡconfected by serumfree medium were markedly higher than that of PBS (free of Ca~ 2+ ,Mg~ 2+ ). There was statistical discrepancy between the two enzyme digestion groups(P<0.05).
结果表明:在获取细胞的数量上,酶消化法明显优于组织块法; 在成活率比较试验中,Ham-F12配制组明显高于常规的PBS液(无Ca2+、Mg2+)配制组,两组之间存在显著差异(P<0.05);收藏指正
4.The explant tissue culture was compared with the typsin digestion culture.
对比了组织块培养和消化培养两种培养方法。收藏指正
5.tumour hospital;
肿瘤医院收藏指正
6.fleshy tumour
7.benign tumour
良性肿瘤收藏指正
8.Studies on Browning Factors of Explant and Proliferation Culture of Walnut(Juglans Regia L.)
核桃外植体褐化影响因素及增殖培养研究收藏指正
9.Advances of explant browning in plant tissue culture
植物组织培养外植体褐变的研究进展收藏指正
10.malignant tumour; cancer;
恶性肿瘤收藏指正
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