universal primer
6.A simple and efficient method, universal primer PCR (UP PCR) is described, that permits preparing DNA probes for FISH from a trace amount of YAC DNA for which sequence does not need to be known. That is, Linking the annealed UP-Linker to the YAC DNA digested with Alu Ⅰ after isolation by pulse field gel electrophresis (PFGE), then amplifying and labelling the YAC DNA by PCR using universal primer (UP).
7.Polymerase Chain Reaction ( PCR) amplification of the internal transcribed spacer (ITS) of the ribosomal DNA of ?Plasmodiophora brassicae? extracting from cruciferae (Chinese cabbage, cauliflower, broccoli) have done with universal primer ITS1 and ITS4, and the PCR products were cloned into pGEM-T vector.
8.5. Based upon the comparison of Cyto b gene sequences in 15 deer species downloaded from GenBank,a universal primer set L15774/HSF21 was used as positive control of the template quality,at the meantime,two species specific primer sets DF/DR and CF/CR were desgined to identify red deer (Cervus elaphus),sika deer (Cervus nippori) and roe deer (Capreolus capreolus) from other species.
