spermary
1.The Establishment of Cell Lines From Spermary of Philosamia cynthia
3.The results showed that the gametogenesis of Yellow sea bream could be divided into five phases and gonad development could be correspondingly divided into six stages. The GSI of ovary at Ⅴphase was 7.60%～13.30%,with average 9.45%,while GSI of spermary atⅤphase was 1.62%～2.71%,with average 1.99%.
4.The result showed that the spermary under 0.05% colchicum for 6-10 hours pretreatment, 0.4% KC1 solution for 45minutes to get the low osmosis, and Giemsa dyed for 10-20 minutes, the clear and separated chromosome can been observed.
