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1.Results The sequence analysis demonstrated that the S cFv of V~{*-~}H and V~{*-~}L chains belong to heavy chain group V(A) and kappa light cha in group IV of mouse immunoglobulin variable region,respectively.
结果 :核苷酸序列分析证明 ,获得的单链抗体基因VH 基因属于小鼠抗体重链可变区基因家族的V(A)组 ,VL 基因属于小鼠抗体κ轻链可变区基因家族的IV组。收藏指正
2.Methods: Complementarity determining region 3(CDR3) size of T cell antigen receptor (TCR) 24 variable region gene was analyzed in peripheral blood lymphocytes (PBL) samples from 16 CML cases using reverse transcriptase-polymerase chain reaction (RT-PCR) and genescan techniques, to identify the distribution and clonality of TCR V P subfamily T cells.
方法:通过RT-PCR技术分析16例CML患者外周血中TCRVβ亚家族T细胞的表达情况,并运用基因扫描技术分析优势表达的T细胞TCRVβ亚家族基因的CDR3长度从而了解其克隆性。收藏指正
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