3.For BPH group and UM+BPH group, after the BPH model were established, Albumin ultrasound contrast agents were injected into every rat from the venacaudalis plus 1MHz, 1w/cm2 ultrasound prostatic local irradiation by the UGT 1025 gene transfection equipment.
4.Methods (1) Kunming mice, weighing 35-45 g, were used. Animal models were made by injection of 0.11mol/L t-BHP (1ml/100g weight) into venacaudalis and after 15 min the animals were put into a closed container (0.3L) for 10 min. Then a stroke index score was tallied for each animal.