3.The diagnostic system has been tested and verified,the measurement results are correct,the results obtained in this paper would be useful for the application of VRM to the slab laser.
5.Results 1.Both GAP-43 ORF gene fragment and the recombinant AAV-EGFP-GAP-43 plasmid were verified by enzyme digestion and DNA sequencing for correct reading frame and orientation.
6.Results: It was verified by PCR and partial nucleotide sequencing that the constructed TIMP?GAA 1 eukaryotic expression vector was correct. After pEGFP-C3 B2?TIMP-1 was transfected into BGC823 cells, the expression of fusion protein was located in the cytoplasm around the karyon.
