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1.The nucleotide sequence homologies of the prevalent virulent strain with China strain, with Brescia strain and with Alfort strain were 81.9%, 83.4% and 83.5% respectively.
结果表明 ,该猪瘟流行毒株的E2核苷酸序列与C株、Brescia株和Alfort株的E2核苷酸序列同源性分别为 81.9%、83.4 %和83.5 % ; 相应地 ,gp5 5氨基酸同源性分别为 94 .8%、95 .6 %和 95 .3%。收藏指正
2.Study on the homology between PRV FB low virulent strain and gE and gI of PA strain
PRV FB弱毒株与FA株gE,gI基因的同源性研究收藏指正
3.The VP2 gene of PPV SY 99 strain was cloned and sequenced. Nucleotide and amino acid identities of VP2 gene between SY 99 strain and NADL 2 strain, Kresse strain, and US 1 strain were conducted. The results showed that SY 99 strain is more closely related to Kresse strain than others and probably a virulent strain.
克隆并测定猪细小病毒 SY- 99株VP2基因 ,与 NADL- 2 ( 4 973)株、NADL- 2( 5 0 75 )株、Kresse株和 US- 1株 VP2基因核苷酸序列及其编码的氨基酸序列进行比较与分析 ,发现 PPV SY- 99株与 Kresse株 VP2基因核苷酸和氨基酸同一性最高 ,推测 SY-99株可能为一强毒株。收藏指正
4.Health experts are worried that a new, more virulent strain may be to blame.
卫生专家考虑这归咎于一种毒力更强的新菌种。收藏指正
5.1. 347 soybean accessions have been identified for the resistance to SMV3by artificially inoculation with SMV 3, a strong virulent strain in NortheastChina.
1．利用人工汁液摩擦法对不同来源的347份大豆种质资源接种SMV3号株系进行抗性鉴定。收藏指正
6.The E?2 gene of prevalent virulent strain of classical swine fever virus was amplified by the RT?PCR and PCR. The amplified fragments was cloned into vector and then sequenced,based on the amino-acid sequences of Alfort and Brescia strains,amino?acid sequence of gp55 protein were deduced. The results showed that there was some differences among recently prevalent virulent strains,C?strain and virulent strains.
利用RT-PCR和PCR技术扩增出近期猪瘟流行野毒株的E2基因,将其克隆到载体上测定核苷酸序列,根据Alfort株和Brescia株、C-株确定起始氨基酸三联体的正确位置后推导出其氨基酸序列,结果表明近期流行的猪瘟毒株与疫苗毒株和强毒株之间存在一定差异;收藏指正
7.This scientific dissertation concentrates on studies on some biological characteristics of Duck Enteritis Virus CH virulent isolate and is summarized as follows:1. Studies on adaptation of Duck Enteritis Virus CH virulent strain (DEV-CHv) to duck embryo fibroblast (DEF) for growth.
本论文对鸭病毒性肠炎病毒CH强毒株(DEV-CHv)部分生物学特性及荧光实时定量PCR对其检测方法的建立和应用进行了研究,主要内容可归纳如下:收藏指正
8.The ICPI in 1-day-old chickens was 1.51,MLD in chicken embryos was 10 -8 ,MDT was 46.2 h,EID 50 in chicken embryos was 10 -10.1 .According to the provided criterion,the results indicated that the avian NDV isolated strain be a virulent strain.
该新城疫病毒分离株对 1日龄雏鸡脑内接种致病指数 (ICPI)为 1.5 1,鸡胚最小致死量 (MLD)为 10 -8,平均死亡时间 (MDT)为 46 .2h ,鸡胚半数感染量 (EID50 )为 10 -10 .1,并与已知标准株进行了比较 . 按规定的标准判定 ,该分离株为毒力较强的鸡新城疫强毒株 .收藏指正
9.5 days after root dip treating,inside of plant alsohad 10~5-10~6 CFU/g tissue of ABPS nOE-104.Before transplant tomato seed-ling in pots,which infested with virulent strain LE-101,roots of seedlingwere dipped in suspension of ABPS nOE-104.nOE-104 have inhibited effectto virulent strain LE-101 on root surface and in seedlings.
浸根处理后5天,nOE-104在番茄体内的数量稳定在10~5～10~6(CHU/克组织)。 番茄苗用拮抗菌nOE-104菌液浸根后移栽,然后再在土壤中添加致病菌LE-101处理的植株,其根表和体内的nOE-104对LE-101的增殖有抑制作用.收藏指正
10.It was reported that newcastle disease virus (NDV) cDNA was succesfully synthesized using synthetic 28 oligonucleotide as primer and using genome UNA of NDV virulent strain as template with reverse transcriptase.
本文报道了用人工合成的28聚寡核苷酸为“引物”,以强毒株新城疫 病毒(NDV)基因组PNA为模板,在逆转录酶作用下成功地合成了NDV- cDNA。 后者经琼脂糖凝胶电泳观察,其大小约为0.5～14kb。收藏指正
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