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1.The results of lauryl sodium sulfate-polyacrylamide gel electrophoreses (SDS-PAGE) of the aggregate precipitate and supernatant and the result of high-performance size-exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi-molecular and tri-molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured-reduced egg white lysozyme; the aggregate precipitate could be further formed by the non-covalent bonds interaction between the soluble hi-molecular egg white lysozyme aggregates, and the soluble tri-molecular egg white lysozyme aggregate could still stay at the supernatant.
沉淀和上清液的不连续十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和高效凝胶排阻层析分析结果表明,还原脲变性蛋溶菌酶在稀释复性过程中除了能够复性成天然态蛋溶菌酶分子外,还会形成可溶的蛋溶菌酶分子二聚体和三聚体,二聚体和三聚体主要是靠分子间二硫键的错配连接而成的;可溶的蛋溶菌酶分子二聚体之间通过非共价键相互作用而形成集聚体沉淀,而可溶的三聚体溶菌酶分子则仍处于复性液上清液中。收藏指正
2.When the denatured reduced egg white lysozymes were renatured by dilution method, an observable amount of aggregate precipitate could be immediately formed.
当用复性液稀释复性还原服变性蛋溶菌酶时,会迅速产生可观量的沉淀。收藏指正
3.Any of various, usually poisonous perennial herbs of the genus Aconitum, having tuberous roots, palmately lobed leaves, blue or white flowers with large hoodlike upper sepals, and an aggregate of follicles.
乌头一种毛茛属通常有毒的多年生草本植物,有管状的根,掌状裂叶,蓝色或色的冠状萼片的花,和骨突的集合收藏指正
4.Characteristics: Climbing perennial woody vines, stem densely hairy; leaves compound, opposite, consisting of three leaflets or sections (ternate), biternate or pinnate, the leaflets 3-9, acuminate at apex, coarsely serrate at margin; inflorescences panicles or cymes, axillary; flowers white to yellowish green, petaloid sepals 4; fruit an aggregate of achenes, anchene with hairy tail.
形态:多年生攀缘性木质藤本,茎被密毛;叶对生,2回3出叶或羽状复叶,小叶3-9,先端渐锐,叶缘粗锯齿;圆锥或聚?花序腋生,花色至淡黄绿色,似花瓣的花萼4片;瘦果聚合,各具毛绒长尾。收藏指正
5.The aggregate of egg white lysozyme molecules formed during the refolding procedure of denatured-reduced egg white lysozymes was analyzed by protein electrophoreses and high-performance size-exclusion chromatography.
摘要本文利用蛋电泳和高效凝胶排阻层析法分析了还原脉变性蛋溶菌酶稀释复性过程中的集聚体。收藏指正
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