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1.(4) Results of pEGFP-N1-GGF2 gene transfection in vivo: The expression of EGFP was observed in the cells of gray matter and white matter around the injected locus by fluorescence microscope in the experimental group, but no expression of fluorescent protein was observed in the control group.
④pECFP-N1-GGF2基因体内转染结果:实验组注射局部脊髓内灰质和白质神经细胞内均有较多绿色荧光蛋白表达; 而对照组未见荧光蛋白表达。收藏指正
2.3. A total of 381 litter records from 127 sows from Duroc, Landrace, Large White and Shanxi White were analyzed to determine whether the different polymorphic locus of PRLR gene influenced total number born and number born alive by using the least square analysis.
3.以杜洛克猪、长白猪、大白猪、山西白猪共127头母猪的381窝产仔记录为实验材料,利用最小二乘分析,研究了PRLR基因各多态位点对猪产仔数性状的影响。收藏指正
3.This thesis gave a study on the polymorphism of ob gene in erhualian, xiang pig,Large White, Duroc, Landrace, Pietran and Landrace X erhualian crossbreeds , by meansof Bi-PASA. Variance analysis showed the correlation of genotypes with litter size andgrowth performance ,and the polymorphism of OB gene locus respectively.
本文采用Bi-PASA方法对二花脸、香猪、大约克、杜洛克、长白、皮特兰和苏钟1系猪七个类型，共计420头猪的DNA样进行了OB基因的分型研究，分析了各基因型之间在产仔数性状和生长性状上的差异，以及各个品种类型在该位点的多态性。收藏指正
4.Based on allelic frequency of various locus the average heterozygosities were caculated: Daroc(d)0.4017, Landrace(L)0.3926, Large white(W)0.3615, LXW 0.4508,WXL 0.4497.DXLW 0.4896, W XLW 0.4611. The results show purebred with the lowest average heterozygosity and other crossbreds with the higher.
根据基因频率计算基因平均杂合度分别为杜洛克0.4017，大白0.3926，长白0.3615，长×大0.4508，大×长0.4497，杜×长大0.4896，大×长大 0.4611。收藏指正
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