2.Objective To establish a transient expression system of mouse DVL-1cDNA recombinant plasmid in cultured wild-type mouse Neuroblastoma 2a (N2a), for the further use in studying the role of Wnt signaling in Alzheimer disease pathogenesis.
3.Methods The cDNA was retrotranscripted from RNA extracted from OFT tissues of both Cx43 knockout and Cx43 wild type mouse embryos on embryonic day (ED) 14.5. The biotin-labeled cRNA derived from the transcription of cDNA was fragmented as probes.
5.Result and conclusion Both TW mouse which carry homozygous wild genes and ATF 1 mouse with half set of wild genes showed the strong tendency of anti-lung tumor characteristics.
6.Methods:Mouse fibroblasts NIH3T3 were transfected separately with three types of retrovirus containing wild type TNF α (Wt TNF); transmembrane TNF α mutant(TM TNFm);
